Unisex public toilets (also referred to as gender-inclusive, gender-neutral, mixed-sex or all-gender, or without any prefix at all) are public toilets that are not separated by gender or sex.

Unisex public toilets take different forms. They may be single occupancy facilities where only one single room or enclosure is provided, or multi-user facilities which are open to all and where users may either share sinks in an open area or each have their own sink in their private cubicle, stall or room. Unisex public toilets may either replace single-sex toilets or may be an addition to single-sex toilets.

Unisex public toilets can be used by people of any sex or gender identity. Such toilet facilities can benefit transgender populations and people outside of the gender binary. Sex-separation in public toilets (also called sex segregation), as opposed to unisex toilets, is the separation of public toilets into male and female. This separation is sometimes enforced by local laws and building codes. Key differences between male and female public toilets in most western countries include the presence of urinals for men and boys, and sanitary bins for the disposal of menstrual hygiene products for women and girls. Sanitary bins may easily be included in the setup of unisex public toilets.

The historical purposes of sex-separated toilets in the United States and Europe, as well as the timing of their appearance, are disputed amongst scholars. Safety from sexual harassment and privacy were likely two main goals of sex-separation of public toilets, and factors such as morality also played roles.^{: 228, 278, 288–89} Paternalism and resistance to women entering the workplace might have also played a role. Some women's groups hold that unisex public toilets will be less safe for women than public toilets that are separated by sex. The push for gender neutral bathrooms is driven by the transgender community to combat harassment and violence against these populations. Unisex public toilets may benefit a range of people with or without special needs (e.g. people with disabilities, the elderly, and anyone who needs the help of someone of another gender or sex), as well as parents who need to help their infant or young child with using the toilet. Advocates say that with the appropriate design interventions, these spaces can improve the safety of all users and reduce the disproportionately long wait times females face in sex-separated public washrooms.